Molecular characterization of a new N-acetylneuraminate synthase (NeuB1) from Idiomarina loihiensis
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منابع مشابه
Molecular Characterization of a Novel N-Acetylneuraminate Lyase from a Deep-Sea Symbiotic Mycoplasma
N-acetylneuraminic acid (Neu5Ac) based novel pharmaceutical agents and diagnostic reagents are highly required in medical fields. However, N-acetylneuraminate lyase（NAL）for Neu5Ac synthesis is not applicable for industry due to its low catalytic efficiency. In this study, we biochemically characterized a deep-sea NAL enzyme (abbreviated form: MyNal) from a symbiotic Mycoplasma inhabiting the st...
متن کاملMolecular characterization of a novel N-acetylneuraminate lyase from Lactobacillus plantarum WCFS1.
N-Acetylneuraminate lyases (NALs) or sialic acid aldolases catalyze the reversible aldol cleavage of N-acetylneuraminic acid (Neu5Ac) to form pyruvate and N-acetyl-d-mannosamine (ManNAc). In nature, N-acetylneuraminate lyase occurs mainly in pathogens. However, this paper describes how an N-acetylneuraminate lyase was cloned from the human gut commensal Lactobacillus plantarum WCFS1 (LpNAL), ov...
متن کاملMolecular Characterization of a Salmonella Typhimurium Isolate from Caspian Pony
Typhoid disease or salmonellosis is a common sickness in horses. In several epidemiological studies inhospitalized horses, several serotypes of Salmonella often are predominant in nosocomial infections.Transportation, overcrowding, dehydration, oral antimicrobial therapy and infections are the risk factorswhich may activate latent or subclinical salmonellosis. In this study, t...
متن کاملPurification, crystallization and characterization of N-acetylneuraminate lyase from Escherichia coli.
N-Acetylneuraminate lyase produced by Escherichia coli was purified and crystallized from a genetically engineered strain (E. coli SF8/pNAL1). The enzyme showed apparent molecular masses of 105,000 Da on gel filtration and 35,000 Da on SDS/PAGE, suggesting that the enzyme is a trimer. The apparent optimum pH and temperature were found to be 6.5-7.0 and 80 degrees C respectively. The Km values f...
متن کاملPurification and properties of N-acetylneuraminate lyase from Escherichia coli.
N-Acetylneuraminate lyase [N-acetylneuraminic acid aldolase EC 4.1.3.3] from Escherichia coli was purified by protamine sulfate treatment, fractionation with ammonium sulfate, column chromatography on DEAE-Sephacel, gel filtration on Ultrogel AcA 44, and preparative polyacrylamide gel electrophoresis. The purified enzyme preparation was homogeneous on analytical polyacrylamide gel electrophores...
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ژورنال
عنوان ژورنال: Glycobiology
سال: 2014
ISSN: 0959-6658,1460-2423
DOI: 10.1093/glycob/cwu096